Spent Media Analysis For Nutrient Utilization and Metabolic Profiling
Non-invasive, time-resolved LC/GC-MS profiling of Culture media to monitor nutrient consumption, byproduct secretion, and bioprocess health.
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Metabolomics of Spent Media for Process Insights
At Clarity Bio Systems, we specialize in high-resolution LC-MS/GC-MS profiling of spent media from microbial and mammalian cultures. Our workflows are optimized for broad coverage of amino acids, organic acids, sugars, SCFAs, and other metabolic markers critical to bioprocess monitoring and media formulation.
Every project includes curated data, annotated results, and expert consultation, helping you interpret metabolic trends, diagnose growth bottlenecks, and guide process or media optimization strategies.
What you get
Spent Media Analysis Panels
Spent Media Analysis is ideal for
Monitoring nutrient
consumption and carbon/
nitrogen source utilization
Tracking secreted
metabolites
Time-course analysis of
metabolic shifts in
bioreactors
Monitoring cell stress,
viability, and metabolic
bottlenecks
Optimization of feed
strategies and media
formulations
Co-culture
interaction studies
Industry Specific Use Cases
Why choose Clarity for metabolomics
10,000+
Samples Analyzed
40+
Publications & patents
10+
Methods
30+
Clients & collaborators
500+
Metabolites
17
Completed Projects
10,000+
Samples Analyzed
40+
Publications & patents
10+
Methods
30+
Clients & collaborators
500+
Metabolites
17
Completed Projects
FAQs
We can analyze chemically defined, complex, and serum-containing media. Typically, 500 µL spent medium per replicate is sufficient for LC-MS or GC-MS workflows.
Yes. We support profiling of samples collected at multiple time points to assess metabolic dynamics across growth or production phases.
It is recommended for interpreting metabolite changes relative to growth or yield.
Yes. We offer integrated analysis to correlate extracellular and intracellular metabolic profiles. However the protocols for both are different. Please talk to us to know more.
Yes. We can analyze VOCs using headspace sampling for additional insight into microbial activity or byproduct formation. We highly recommend in-house sample preparation specially for VOCs.
Get in touch to explore how metabolomics of spent media can improve your media design, productivity, and process efficiency.